Present and future of quantitative developmental biology

Seminar organized by Jean-Léon Maître from 25 to 29 July 2022

 

Participants

Mariaceleste Aragona (Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)/ University of Copenhagen, Denmark), Elias Barriga (Instituto Gulbenkian de Ciencia, Oeiras, Portugal), Tommaso Cavazza (MPG, Germany), Romain Levayer (Institut Pasteur, Paris), Jean-Léon Maître (Institut Curie, Paris, France), Rita Mateus (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany), Kate McDole (MRC Laboratory of Molecular Biology, Cambridge, UK), Renaud Pourpre (Journalist, L’Exploratoire), Rashmi Priya (The Francis Crick Institute, London, UK), Anne-Cécile Reymann (Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Paris, France), Verena Ruprecht (Centre for Genomic Regulation (CRG), Barcelona, Spain), Timothy Saunders (University of Warwick, UK), Jakub Sedzinski (Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Copenhagen, Denmark), Ina Sonnen (Hubrecht Institute, Utrecht, the Netherlands).

 

Résumé

Human blastocyst with cells in red and their nuclei in blue. ©Julie Firmin et Jean-Léon Maître.

Quelle époque captivante pour étudier le développement embryonnaire ! L’émergence de systèmes expérimentaux, de méthodes et d’analyses permet de poser de nouvelles questions ou d’en revisiter d’anciennes. En particulier, l’étude du patterning et de la morphogénèse, qui vise à comprendre comment l’embryon établit le plan du corps en positionnant les cellules aux destins cellulaires et aux formes adéquates, bénéficie de rapides transformations technologiques et conceptuelles. Par exemple, microfluidique et microfabrication permettent de manipuler les conditions de culture des embryons avec une plus grande flexibilité ; les systèmes organoïdes reconstituent des tissus et autres structures complexes simplement à partir de cellules souches ; les méthodes de microscopie telles que la microscopie à feuillets de lumière, les senseurs fluorescents ou autres outils optogénétiques permettent de disséquer des mécanismes développementaux sur des échelles spatiotemporelles jamais vues. Ces possibilités nouvelles seront centrales pour structurer la communauté scientifique durant les prochaines décennies. Cette recherche sera menée en partie par une nouvelle génération de scientifiques. Du 25 au 29 juillet 2022, des scientifiques ayant récemment établis leur équipe de recherche en Europe se sont rassemblés aux Treilles pour discuter l’émergence des approches quantitatives pour l’étude du développement embryonnaire. Embryons de nématode, mouche, oursin, grenouille, poisson zèbre, vache, souris et humain étaient au programme pour discuter de processus développementaux allant de la fertilisation à l’organogénèse.

 

Abstract

What an exciting time to be studying embryonic development! Emerging experimental systems, methods and analyses allow addressing a whole new set of fascinating questions, as well as revisit older ones. In particular, the field of patterning and morphogenesis, which investigates how embryos establish their body plan with the correct cell types and shape, currently experiences rapid technological and conceptual transformations. For example, microfluidics and microfabrication now permit manipulating the culture conditions of developing embryos with extended flexibility; organoid systems allow the reconstitution of tissues and structures entirely from stem cells; microscopy methods such as light sheet microscopy, live sensor of signalling pathways, optogenetic tools allow dissecting developmental mechanisms over unprecedented temporal and spatial scales. Together, these new possibilities will be key to structure the field of patterning and morphogenesis over the next decades. This research will be carried out, in part, by a new generation of emerging leaders. From July 25th to 29th of 2022, scientists who recently established their labs in Europe gathered in Les Treilles to discuss the emerging quantitative approaches to study embryonic development. From nematodes, flies, sea urchin, frogs, zebrafish, cow and mouse to human, a broad variety of developmental processes were covered from fertilization to organogenesis.

 

Observing embryonic development is key to understand it.

Technological developments in light sheet microscopy have allowed imaging of large-scale morphogenetic events while maintaining cellular resolution. Kate McDole, who locked herself in a room during the pandemic to build a new light sheet microscope, presented never-seen-before cell movements during mouse gastrulation, shedding light on the origin of cells constituting the different germ layers. Rita Mateus also used light sheet microscopy to image the patterns involved in the formation of the zebrafish pectoral fins and better characterise how those patterns scale during growth and regeneration. As the fins emerge, other organs also form convoluted structures such as the trabeculae that contract the ventricle of the heart. Rashmi Priya further showed how high-resolution microscopy and clonal analysis help understand the origin of tissue complexity. Zebrafish was also at the heart of Diana Pinheiro’s work, as she reported on a mechanism explaining the coordination of mesoderm progenitors migration and specification by Nodal signaling during gastrulation. When missing, Nodal signaling causes characteristic phenotypes that expert zebrafish embryologists can easily recognize. Patrick Müller leveraged deep learning analyses to develop algorithms that outperform embryologists at identifying the phenotypes of zebrafish gastrulation mutants.

Together, these studies show how imaging large-scale shape changes and quantitatively tracking them can help understand the global patterning and morphogenesis of embryos.

Down to the sub-cellular scale, high resolution microscopy reveals the mechanisms employed by cells to shape embryos. In particular, Anne-Cécile Reymann looked into the influence and distribution of maternally deposited regulators of the actin cytoskeleton during the early cleavages of c elegans embryos. In contrast, Tommaso Cavazza reported on how microtubules help coordinate chromosome segregation in cow zygotes before the nuclear envelope of the parental pronuclei breaks down. Later during mammalian development, Jean-Léon Maître described how protrusions help cells control the formation and positioning of a fluid-filled lumen that helps setting the first axis of symmetry of the embryo. A different set of protrusions was introduced by Kuba Sedzinski to explain how cells can insert themselves within epithelia and increase the complexity of tissues. Finally, peering into fly embryos, Timothy Saunders quantified the scaling of Bcd gradients by combining fluorescence correlation spectroscopy and mutants affecting egg size.

Altogether, bridging the sub-cellular scale to embryonic changes is key to understand the molecular and physical regulation of embryonic development.

 

Probing and challenging embryos in novel ways

Exploring how cells change their chemical composition and physical properties during development will help understand their behaviour. Mariaceleste Aragona quantitatively analysed the effect of tissue stretching in vivo using clonal analysis and single cell sequencing to understand how cells change composition and fate after a long-term physical perturbation. To understand how cells sense mechanical perturbations, Verena Ruprechtinvestigated the short-term response of cells to compression by combining high resolution microscopy, microfabrication and optical tweezers. Tweezers were also on the menu for Nicolas Minc who developed magnetic tweezers strong and precise enough to move the entire mitotic spindle within cells to induce division asymmetries and explore the mechanics of the cytoplasm.

Understanding how embryos develop requires the need to finely perturb this process in controlled ways. Romain Levayer leveraged the powerful genetics of Drosophila and optogenetics to induce the death of a precise number of cells and determine the range of robustness of epithelia to cell delamination. Another way to control in space and time the molecular regulation of embryonic development is microfluidics: using precise oscillations of chemical compound, Ina Sonnen could explore the coupling of biological clocks during somitogenesis. Finally, Elias Barriga measured endogenous electric fields and applied electric fields of similar magnitude to steer the migration of neural crest cells in Xenopus embryos.

Together, discussing the current research and future projects of young European scientists revealed the promises and challenges of applying quantitative methods to developmental biology. On the one hand, quantitative methods allow dissecting developmental mechanisms with unprecedented precision. On the other hand, the integration of multiple spatial and temporal scales, and different source of biological information (such as chemical, mechanical and electrical signals) remain extremely challenging. Moreover, tackling these problems when most biologists lack the appropriate training in mathematics and coding can be challenging. A consensus appeared regarding the need to include more biology-oriented maths in university programmes, as this will be key to anchor biology to the realm of modern science. Importantly, Renaud Pourpre, who carried out several initiatives to bring microscopy data to the public, reminded us how microscopy images constitute a straightforward avenue to communicate around science to a lay audience. By the same token, there is no doubt that the beauty of developing embryos will continue motivating biologists and other scientists to expand their toolbox to decipher the mechanisms of development.

 


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